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醫(yī)學(xué)免費(fèi)論文:鞘內(nèi)七葉皂苷和可樂(lè)定混合的協(xié)同抗炎癥痛作用
【摘要】 探討蛛網(wǎng)膜下腔(鞘內(nèi))七葉皂苷及混合可樂(lè)定對(duì)甲醛炎癥痛的治療作用�！痉椒ā� 雄性SD大鼠(250 ～ 300 g)隨機(jī)分為對(duì)照組、七葉皂苷組、可樂(lè)定組、混合藥物組、拮抗組。蛛網(wǎng)膜下腔置管后注射實(shí)驗(yàn)藥物，制作甲醛炎癥痛模型，觀察大鼠行為學(xué)改變情況，記錄60 min內(nèi)每5 min的退縮反應(yīng)時(shí)間(s)，采用線性回歸的方法計(jì)算各藥物的半數(shù)有效劑量(ED50)及其95%可信區(qū)間(CI95)，用等輻射法評(píng)價(jià)藥物的相互作用�！窘Y(jié)果】 鞘內(nèi)單獨(dú)注射七葉皂苷或可樂(lè)定可產(chǎn)生劑量依賴(lài)性的抗炎癥痛作用;鞘內(nèi)七葉皂苷和可樂(lè)定混合，可以產(chǎn)生明顯的協(xié)同效應(yīng);鞘內(nèi)育亨賓預(yù)處理不能拮抗鞘內(nèi)七葉皂苷的抗炎癥痛作用，而可樂(lè)定及七葉皂苷混合可樂(lè)定的抗炎癥痛作用可被育亨賓拮抗或部分拮抗�！窘Y(jié)論】 鞘內(nèi)單獨(dú)注射七葉皂苷或可樂(lè)定可產(chǎn)生劑量依賴(lài)性抗炎癥痛作用;鞘內(nèi)注射七葉皂苷能增強(qiáng)可樂(lè)定的抗炎癥痛作用醫(yī).學(xué).全.在.線m.gydjdsj.org.cn。
【關(guān)鍵詞】 甲醛; 炎癥痛; 七葉皂苷; 可樂(lè)定; 大鼠
Synergistic Effects between Intrathecal Escin and Clonidine on Treatmentf Inflammatory Pain in Rats LI Qiang， OUYANG Han-dong， HE Wei-xiong， ZENG Wei-an 1. Guangzhou Medical College Cancer Hospital， Guangzhou， 510095; 2. State Key Laboratory of Oncology in SouthernSun Yat-sen University Cancer Center， Guangzhou 510060， ChinaAbstract： 【Objective】 To investigate the effects of intrathecal escin and its interaction with clonidine on treatment of inflammatory pain induced by formaldehyde injection. 【Methods】 Male SD rats weighing 250 ～ 300 g were implanted with chronic lumbar intrathecal catheters， and were randomly divided into： group control; group escin; group clonidine; group escin + clonidine and group yohimbine (pretreatment with intrathecal yohimbine before intrathecal administration of escin， clonidine and a mixture of escin and clonidine). The formaldehyde test was used as a model of nociception. Antinociceptive effect was assessed by Biphasic painful behavior (flinch time per 5 min). The dose-response lines were fitted using linear regression analysis， and ED50 values and their 95% confidence intervals (CI95) were calculated. Isobolographic analysis of the escin and clonidine interaction was conducted. 【Result】 Intrathecal escin dose-dependently suppressed the flinch during phase Ⅱ in formaldehyde test. The ED50 of escin alone was 16.71 μg in phase Ⅱ. Intrathecal clonidine produced dose-dependent suppression of the biphasic responses in formaldehyde test. The ED50 of clonidine alone was 4.17 μg in phase Ⅰ and 2.58 μg in phase Ⅱ. Isobolographic analysis showed that the combination of intrathecal escin and clonidine synergistically reduced flinching activity in phase Ⅱ. The experimental ED50 of combination was significantly lower than the theoretical ED50. Intrathecal pretreatment with yohimbine attenuated the effects of clonidine and the combination of escin and clonidine， but antinociception of escin cannot be antagonized by pretreatment of yohimbine. 【Conclusion】 Intathecal escin and clonidine alone produced a dose-dependent treatment effects on inflammatory pain. The enhancing effects were found between intrathecal escin and clonidine.
Key words： formaldehyde; inflammatory pain; escin; clonidine; rats
炎癥痛在臨床廣泛存在，目前常用非甾體類(lèi)抗炎鎮(zhèn)痛藥(NSAID)或阿片類(lèi)藥物進(jìn)行治療，但NSAID 類(lèi)的消化道不良反應(yīng)及心血管風(fēng)險(xiǎn)限制了它們的應(yīng)用[1-2]。而阿片類(lèi)藥物的便秘、藥物耐受情況在長(zhǎng)期用藥時(shí)發(fā)生率極高。因此，臨床迫切需要抗炎癥痛新藥及協(xié)同治療方法。七葉皂苷(Escin)屬三萜皂苷類(lèi)化合物，具有抗炎、抗水腫及增加靜脈張力的作用[3]，但是否具有抗炎癥痛作用未見(jiàn)報(bào)道。本研究通過(guò)制作大鼠甲醛炎癥痛蛛網(wǎng)膜下腔(鞘內(nèi))給藥模型，評(píng)價(jià)了七葉皂苷及混合可樂(lè)定的抗炎癥痛作用醫(yī).學(xué).全.在.線m.gydjdsj.org.cn。
