醫(yī)學(xué)免費(fèi)論文:誘導(dǎo)的視網(wǎng)膜色素上皮細(xì)胞增生作用及其作用機(jī)制

醫(yī)學(xué)免費(fèi)論文:誘導(dǎo)的視網(wǎng)膜色素上皮細(xì)胞增生作用及其作用機(jī)制

【摘要】 目的：觀察(R, R)�瞂Y��10和(S, S)�瞂Y��10對(duì)視網(wǎng)膜色素上皮細(xì)胞的增生作用，并且進(jìn)一步的研究其作用機(jī)制。但(R, R)�瞂Y��10和(S, S)�瞂Y��10對(duì)人臍靜脈內(nèi)皮細(xì)胞并無(wú)增生的作用。方法：通過(guò)人視網(wǎng)膜色素上皮細(xì)胞(ARPE��19)和人臍靜脈內(nèi)皮細(xì)胞(HUVECs)研究(R, R)�� XY��10和(S, S)�瞂Y��10對(duì)視網(wǎng)膜色素上皮細(xì)胞的增生作用，并且采用ERK、KT、PI3K、蛋白激酶C(PKC)和一氧化氮合酶(NOS)抑制劑來(lái)研究其作用機(jī)制。結(jié)果：(R, R)�瞂Y��10和(S, S)�瞂Y��10促進(jìn)了ARPE��19細(xì)胞的增生，并具有劑量依賴(lài)性，但是對(duì)HUVECs細(xì)胞沒(méi)有影響。如果同時(shí)加入增生抑制劑H7 (5μmol/L)、金絲桃素(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH��5(10μmol/L)和L�睳AME (100μmol/L)，則給予H7、金絲桃素、PD98059和LY294002各組的增生作用受到了抑制，而給予SH��5和L�睳AME兩組的增生作用沒(méi)有影響。結(jié)論：(R, R)�瞂Y��10和(S, S)�瞂Y��10能夠誘導(dǎo)ARPE��19細(xì)胞增生，其作用可能是通過(guò)MAPK和PI3K的途徑來(lái)發(fā)揮該作用。因此，(R, R)�瞂Y��10和(S, S)�瞂Y��10能通過(guò)修復(fù)損傷的RPE細(xì)胞來(lái)治療老年性黃斑變性。

【關(guān)鍵詞】 年齡相關(guān)性黃斑變性;(R,R)�瞂Y��10; (S,S)�瞂Y��10; ARPE��19 cells;人臍靜脈血管內(nèi)皮細(xì)胞;增生

Controlled continuous curvilinear capsulorhexis in short axial length and shallow anterior chamber eyes

Guang�瞃u Yang, You�睱i Huang, Zhi�睩eng Wu 醫(yī).學(xué).全.在.線m.gydjdsj.org.cn

Department of Ophthalmology, the Second People�餾 Hospital,Wuxi 214002,Jiangsu Province,China

Correspondence to:Guang�瞃u Yang.Department of Ophthalmology, the Second People�餾 Hospital,Wuxi 214002,Jiangsu Province,China.wuxiygy@163.com

Abstract�rAIM：To investigate the efficacy of controlled continuous curvilinear capsulorhexis(CCC) technique in short axial length and shallow anterior chamber eyes.�rMETHODS：Sixty�瞖ight patients(68 eyes) with short axial length and shallow anterior chamber were included.The routine CCC technique was used in 32 cases (32 eyes) and controlled CCC technique was used in 36 cases (36 eyes).The success rate and complication were compared between two groups.�rRESULTS：The success rate of the routine technique group and controlled technique group was 53.13% and 86.11% respectively. Incomplete CCC leading to posterior capsule tears was 9.38% and zero in two groups respectively.�rCONCLUSION: Controlled CCC technique can increase the success rate and reduce complications in short axial length and shallow anterior chamber eyes.

�r

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