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醫(yī)學(xué)免費(fèi)論文:豚鼠銀屑病樣動(dòng)物模型中角蛋白K14，16，17表達(dá)水平的變化
關(guān)鍵詞: 銀屑病;角蛋白;疾病模型，動(dòng)物
中圖號(hào):R758.63
摘 要:目的 研究心得安外涂所致豚鼠銀屑病樣動(dòng)物模型皮損中角蛋白K14，16，17表達(dá)水平的變化，從分子水平研究此種動(dòng)物模型的可行性. 方法 用50g L-1 心得安乳劑外涂豚鼠耳背部皮膚誘導(dǎo)銀屑病樣動(dòng)物模型，應(yīng)用原位雜交法檢測(cè)銀屑病樣皮損中角蛋白K14，16，17的表達(dá). 結(jié)果 心得安外涂所致豚鼠銀屑病樣動(dòng)物模型皮損中角蛋白K14，16表達(dá)水平較正常對(duì)照組明顯增高，K17的水平無明顯變化. 結(jié)論 心得安外涂所致豚鼠銀屑病樣動(dòng)物模型不僅在組織病理學(xué)而且在分子水平上與人類銀屑病皮損基本一致.
Study of Keratin14，16，17expression in lesions of guinea pig psoriasis┐like animal model
FAN Ping-Shen，LIAO Wen-Jun，LIU Yu-Feng
Department of Dermatology，Xijing Hospital，F(xiàn)ourth Military Medical University，Xi'an710033，China
Keywords:psoriasis;keratin;disease models，animal醫(yī).學(xué).全.在.線m.gydjdsj.org.cn
Abstract:AIM To investigate the changes of keratin14，16，17expression in lesions of guinea pig's psoriasis-like ani-mal model caused by topical treatment with propranolol and explore the feasibility of this animal model at molecular level.METHODS Psoriasis-like animal model was established by topical treatment with5%propranolol emulsion on ear's back of guinea pig.Expression of keratin14，16，17in lesions were detected by in situ hybridization.RESULTS Expres-sion of keratin14，16in lesions of treated group were signifi-cantly increased，while expression of K17was not significant-ly different with that in control group.CONCLUSION Guinea pig's psoriasis-like animal model caused by topical treatment with propranolol has high concordance with human psoriatic lesions not only in histopathology，but at molecular level.
引言
銀屑病的發(fā)病機(jī)制極其復(fù)雜，其組織病理學(xué)的改變主要表現(xiàn)為角質(zhì)形成細(xì)胞過度增殖、炎癥細(xì)胞聚集和真皮乳頭部血管擴(kuò)張三大特征.其中，角質(zhì)形成細(xì)胞過度增殖常常伴有異常的角化過程，同時(shí)出現(xiàn)角蛋白表達(dá)的改變.為了能夠更好地研究銀屑病的發(fā)病機(jī)制，合適而科學(xué)的動(dòng)物模型顯得極為重要.我們從分子水平研究心得安外涂所致豚鼠銀屑病樣動(dòng)物模型的可行性.
